A method of culturing Antrodia cinnamomea with high Triterpenoids includes mixing a malt extract, glucose, peptone, agar, and a mushroom's extract with distilled water to obtain a culture medium; providing the culture medium to a high-pressure sterilization machine for sterilization to obtain a sterilized culture medium; providing the sterilized culture medium to an incubator in a disinfection environment to obtain a solidified culture medium after a predetermined time; transplanting Antrodia cinnamomeas strains to the solidified culture medium at separated positions; and exposing the solidified culture medium under a beam.