The invention provides a groove structure for printing coating process and manufacturing method thereof. With the groove structure as an interpenetrating and co-axial stacked structure of a first and second grooves formed respectively by first causeway and second causeways, and the smallest part of the second groove opening greater than the largest part of the first groove opening; the inclining inner circumferential surface of the first groove hydrophilic, while the upper surface of the first causeway, the inclining inner circumferential surface of the second groove, and the upper surface of the second causeway hydrophobic, the present invention is able to increase the size of the groove opening for printing coating process without reducing the pixels per inch so that the ink drops can be more easily dripped into the groove. As such, the invention reduces the demand on printer precision and manufacturing difficulty to improve competitiveness.