Provided is a manufacturing method of a sheet having needle-like protrusions capable of individually manufacturing sheets on a mold. The manufacturing method of a sheet having needle-like protrusions includes, for a mold provided with needle-like recesses and an annular groove provided around a region where the needle-like recesses are formed, a first polymer solution supplying step of supplying a first polymer solution, which is to become a first layer, to the needle-like recesses; a second polymer solution supplying step of filling the needle-like recesses and the groove with a second polymer solution by supplying the second polymer solution, which is to become a second layer, to a surface of the mold; a drying step of forming a laminate of the first layer and the second layer by drying the first polymer solution and the second polymer solution; and a peeling step of peeling the laminate away from the mold.