- 专利标题: METHOD OF ENRICHING AND DETECTING A TARGET MICROORGANISM
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申请号: US15756100申请日: 2016-08-29
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公开(公告)号: US20180258458A1公开(公告)日: 2018-09-13
- 发明人: GREGORY W. SITTON
- 申请人: 3M INNOVATIVE PROPERTIES COMPANY
- 国际申请: PCT/US2016/049182 WO 20160829
- 主分类号: C12Q1/04
- IPC分类号: C12Q1/04 ; G01N33/58 ; G01N21/64
摘要:
The present disclosure provides a method of detecting a target bacterium. The method includes forming a primary enrichment culture that includes a test sample, a primary enrichment medium and a redox dye; incubating the primary enrichment culture for a first period of time equal to eight hours or less; assessing the primary enrichment culture during the first period of time to detect whether the redox dye changes from a first state to a second state; if the second state is detected in the primary enrichment culture during the first period of time, mixing a portion of the primary enrichment culture with a secondary enrichment medium to form a secondary enrichment culture and incubating the secondary enrichment culture for a second period of time; and performing a test to detect the target bacterium using a portion of the second enrichment culture.