The invention provides a time-division multispectra detection device and method. The time-division multispectra detection device includes a plurality of light-emitting units of different wavelengths sequentially activatable by a control unit to emit one same wavelength of light onto an object at one same time. When one light-emitting unit is activated, the control unit synchronously activates the digital image sensing unit to obtain an image data of the object corresponding to one single wavelength of light from one respective light-emitting unit. After every spectrum image data is collected. A judgment of the human physiological characteristics of the object is performed, effectively reducing the overall costs.