- 专利标题： KIT FOR DETECTING ctDNA AND EXPRESSION OF DRUG-RESISTANT GENES RELATED TO APPLICABILITY OF TUMOR CHEMOTHERAPEUTIC DRUGS
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申请号： US15944725申请日： 2018-04-03
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公开(公告)号： US20190300961A1公开(公告)日： 2019-10-03
- 发明人: WENFENG YU , SHI ZHOU , ZHI HUANG , BI WANG , RUI GAO , DAI WEI ZHAO , HAI ZHUANG , YA LING LIANG
- 申请人： GUIZHOU MEDICAL UNIVERSITY , MATERNAL AND CHILD HEALTH HOSPITAL OF GUIYANG CITY , GUIZHOU ENTRY-EXIT INSPECTION AND QUARANTINE BUREAU
- 专利权人： GUIZHOU MEDICAL UNIVERSITY,MATERNAL AND CHILD HEALTH HOSPITAL OF GUIYANG CITY,GUIZHOU ENTRY-EXIT INSPEC
- 当前专利权人： GUIZHOU MEDICAL UNIVERSITY,MATERNAL AND CHILD HEALTH HOSPITAL OF GUIYANG CITY,GUIZHOU ENTRY-EXIT INSPEC
- 主分类号： C12Q1/6886
- IPC分类号： C12Q1/6886 ; C12Q1/6869 ; C12Q1/686 ; C07D227/02
摘要：
A kit for determining ctDNA concentration and a method for determining ctDNA concentration is disclosed, wherein the method comprising dissolving extracted ctDNA in Tris-HCl buffer with pH>7.0 and adding into the buffer 2,7-bis(1-methyl-4-vinylpyridine)-9-ethylcarbazole iodised salt as a molecular probe, calculating the concentration of ctDNA in the solution by measuring the Fluorescence intensity of the solution. It has extremely high sensitivity in the determination of 0 μg/ml˜50 μg/ml ctDNA solution.
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