The invention provides a method for regulation of a sample manipulator. The method includes generating a list of at least one first displacement for at least one pre-defined object; estimating a least one second displacement with respect to at least one location on the manipulator; comparing the estimated displacement with the list of first displacements to obtain an optimum displacement; and computing at least one second force at the given location from the obtained optimum displacement. The estimation of the optimum displacement facilitates regulation of the manipulator.