The antiseptic wound dressing includes at least nanometer chitin, alkaline earth metal alginate, and an antiseptic material. The nanometer chitin is tubular shaped having diameter 10˜50 nm and length 20˜200 nm. The amount of the nanometer chitin is 0.1%˜5% to the alkaline earth metal alginate. The antiseptic wound dressing is manufactured by mixing nanometer chitin and alkaline earth metal alginate, further mixing with antiseptic material, conducting a wet spinning process to produce fibers, and conducting a non-woven cloth process to obtain the antiseptic wound dressing of the present invention. Therefore, the antiseptic wound dressing is capable of reducing the chance of would infection, and providing superior moisture retention and enhanced wet strength.