- 专利标题： Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
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申请号： US15998679申请日： 2018-08-16
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公开(公告)号： US20200002740A9公开(公告)日： 2020-01-02
- 发明人: Lyle J. Arnold , Norman C. Nelson
- 申请人： Lyle J. Arnold , Norman C. Nelson
- 申请人地址： US CA Poway
- 专利权人： Aegea Biotechnologies, Inc.
- 当前专利权人： Aegea Biotechnologies, Inc.
- 当前专利权人地址： US CA Poway
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C12Q1/6844
摘要：
The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
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