公开(公告)号：US11639518B2

公开(公告)日：2023-05-02

申请号：US17584959

申请日：2022-01-26

申请人： President and Fellows of Harvard College

发明人： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/53

摘要： Methods of analyzing nucleic acids of a cell are provided.

公开(公告)号：US20230127948A1

公开(公告)日：2023-04-27

申请号：US17798248

申请日：2021-02-12

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Jonathan Gootenberg ,  Omar Abudayyeh ,  Julia Joung ,  Alim Ladha ,  Han Altae-Tran ,  Guilhem Faure ,  Feng Zhang

IPC分类号： C12Q1/6844 ,  C12N9/22 ,  C12Q1/70

摘要： Systems and methods for rapid diagnostics related to the use of CRISPR effector systems and optimized guide sequences for detection of coronavirus, including multiplex lateral flow diagnostic devices and methods of use, are provided.

公开(公告)号：US20230091060A1

公开(公告)日：2023-03-23

申请号：US17790277

申请日：2021-02-05

申请人： University of Conecticut

发明人： Changchun LIU ,  Kun YIN

IPC分类号： C12Q1/6844 ,  C12Q1/6806 ,  C12N9/12

摘要： Described herein is an aqueous, miscible, multiphase, one-pot detection system including a first phase comprising a low density solution comprising a nucleic acid detection system; and a second phase in diffusive communication with the first phase, the second phase having a higher density than the first phase, and the second phase including a nucleic acid amplification system. Also included are multiwell plates and/or devices including the system and methods of detecting target nucleic acids.

公开(公告)号：US20230077364A1

公开(公告)日：2023-03-16

申请号：US17991309

申请日：2022-11-21

申请人： 10x Genomics, Inc.

发明人： David Michael Patterson ,  Sultan Doganay Tuncer ,  Zachary Bent

IPC分类号： C12Q1/6837 ,  C12N9/12 ,  C12N9/00 ,  C12Q1/6813 ,  C12Q1/6844

摘要： The present disclosure provides methods and compositions for detecting and spatially locating analyte interactions and gene expression in a biological sample. For example, provided herein are methods of determining a location of at least one analyte in a biological sample using analyte-binding moieties, proximity ligation, and an array including capture probes.

公开(公告)号：US11602752B2

公开(公告)日：2023-03-14

申请号：US16310527

申请日：2017-06-30

申请人： SEEGENE, INC.

发明人： Jong Yoon Chun ,  Seung Jae Lee

IPC分类号： B01L7/00 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/6816 ,  C12Q1/6844 ,  G01N21/64 ,  G01N21/03

摘要： The present invention relates to an apparatus for performing a nucleic acid amplification reaction and a fluorescence detection device for reaction analysis. The nucleic acid amplification apparatus of the present invention uses a plurality of blocks having different reaction temperatures by independent temperature control and the movement between the blocks is performed along sliding recesses formed in the blocks, enabling to greatly shorten the total amplification time (TAT). In the fluorescence detection device of the present invention, the positions of the light source and the photodetector are very unique for the reaction vessel in which an excitation light is provided and an emission light is generated.

公开(公告)号：US20230070234A1

公开(公告)日：2023-03-09

申请号：US17986873

申请日：2022-11-14

申请人： Bio-Rad Laboratories, Inc.

发明人： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC分类号： C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01F23/41

摘要： Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11591649B2

公开(公告)日：2023-02-28

申请号：US16796748

申请日：2020-02-20

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  Matthew Hill ,  Bernhard Zimmermann ,  Johan Baner ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Styrmir Sigurjonsson ,  Zachary Demko

IPC分类号： C12Q1/6869 ,  G16B10/00 ,  C12Q1/6876 ,  C12Q1/6844

摘要： Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

公开(公告)号：US11591644B2

公开(公告)日：2023-02-28

申请号：US16480521

申请日：2018-01-25

申请人： Sense Biodetection Limited

发明人： Henry John Lamble ,  David Lloyd ,  Christopher Egan

IPC分类号： C12Q1/6844 ,  C12Q1/682

摘要： The present invention relates to methods for the detection of nucleic acids of defined sequence and kits for use in said methods. The methods employ nicking agent(s), polymerase and oligonucleotide probes to produce probe fragments in the presence of a target nucleic acid.

公开(公告)号：US20230057343A1

公开(公告)日：2023-02-23

申请号：US17855199

申请日：2022-06-30

申请人： Bio-Rad Laboratories, Inc.

发明人： Duc DO ,  Claudia LITTERST ,  Dianna MAAR

IPC分类号： C12Q1/686 ,  C12Q1/6844 ,  G01N21/64

摘要： Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US11578322B2

公开(公告)日：2023-02-14

申请号：US16867256

申请日：2020-05-05

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC分类号： C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

摘要： A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.