- 专利标题： RAPID DETECTION OF ZIKA VIRUS BY REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
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申请号： US16609994申请日： 2018-04-27
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公开(公告)号： US20200063216A1公开(公告)日： 2020-02-27
- 发明人: Amanda E. Calvert
- 申请人： The U.S.A., as represented by the Secretary, Department of Health and Human Services
- 申请人地址： US MD Bethesda
- 专利权人： The U.S.A., as represented by the Secretary, Department of Health and Human Services
- 当前专利权人： The U.S.A., as represented by the Secretary, Department of Health and Human Services
- 当前专利权人地址： US MD Bethesda
- 国际申请： PCT/US2018/029738 WO 20180427
- 主分类号： C12Q1/6888
- IPC分类号： C12Q1/6888 ; C12Q1/70 ; C12Q1/6844
摘要：
A highly sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect ZIKV nucleic acid in biological samples is described. The disclosed assay is capable of detecting as few as one RNA copy per μl and can be performed in a clinical or field setting with minimal equipment and technological expertise. Oligonucleotide primers and kits for detecting ZIKV nucleic acid are also described.
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