- 专利标题: SINGLE LIGHT SOURCE, TWO-OPTICAL CHANNEL SEQUENCING
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申请号: US16468269申请日: 2018-03-06
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公开(公告)号: US20200080142A1公开(公告)日: 2020-03-12
- 发明人: Robert LANGLOIS , John VIECELI , Xiaohai LIU
- 申请人: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
- 申请人地址: US CA San Diego GB Nr Saffron Walden, Essex
- 专利权人: Illumina, Inc.,Illumina Cambridge Limited
- 当前专利权人: Illumina, Inc.,Illumina Cambridge Limited
- 当前专利权人地址: US CA San Diego GB Nr Saffron Walden, Essex
- 国际申请: PCT/US2018/021055 WO 20180306
- 主分类号: C12Q1/6869
- IPC分类号: C12Q1/6869 ; G01N21/64
摘要:
Disclosed is a system for determining the nucleotide sequence of polynucleotides. The system can comprise a light source, such as a laser or a LED, configured to generate light at a predetermined wavelength. A detector of the system can detect fluorescent emissions at a first wavelength and a second wavelength. A processor of the system identify the nucleotide as a first type if no fluorescent emission is detected by the at least one detector; identify the nucleotide as a second type if a fluorescent emission at the first wavelength of light is detected by the at least one detector; identify the nucleotide as a third type if a fluorescent emission at the second wavelength of light is detected by the at least one detector; and identify the nucleotide as a fourth type if fluorescent emissions at the first wavelength and the second wavelength of light are detected by the at least one detector.