- 专利标题: METHODS OF ASSAYING TROPOLONE
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申请号: US16769842申请日: 2018-12-04
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公开(公告)号: US20210178289A1公开(公告)日: 2021-06-17
- 发明人: Benjamin Alexander WILKES
- 申请人: LONZA LTD
- 申请人地址: CH Visp
- 专利权人: LONZA LTD
- 当前专利权人: LONZA LTD
- 当前专利权人地址: CH Visp
- 国际申请: PCT/US2018/063822 WO 20181204
- 主分类号: B01D15/08
- IPC分类号: B01D15/08 ; C07K16/00 ; C07C49/717
摘要:
Disclosed herein are methods useful for detecting and/or quantifying tropolone in samples, e.g., generated during the production of and in final formulations of a product, e.g., a recombinant protein, e.g., an antibody. Tropolone and derivatives thereof (cycloheptatriene ketones) are separated from a mixture by adding a partially or fully fluorinated alkyl or aryl (e.g. pentafluorophenyl-propyl), which (covalently) binds the tropolone-like compound. Then it is assayed via UV or tandem mass spectrometry. A reaction mixture of tropolone-like compounds and fluorinated alkyl or aryl is also disclosed.