Disclosed are a micropore-filled amphoteric membrane for low vanadium ion permeability, a method of manufacturing the same, and a vanadium redox flow battery including the amphoteric membrane. The micropore-filled amphoteric membrane for low vanadium ion permeability minimizes crossover of vanadium ions, which occurs between a catholyte and an anolyte in a redox flow battery, and has low membrane resistance and thus has remarkably improved performance as compared to commercially available ion-exchange membranes such as Nafion, and accordingly, can be effectively used in the manufacture of a redox flow battery. In addition, the micropore-filled amphoteric membrane is continuously manufactured through a roll-to-roll process, and thus the manufacturing process is simple and manufacturing costs can be greatly reduced.