This optical fiber alignment jig for aligning a plurality of optical fibers with the tip end coating stripped off to expose glass fiber includes a rail; a convex push-up part capable of moving in the extending direction of the rail; and a plurality of plate-shaped parts that each have a first surface and a second surface perpendicular to the extending direction of the rail and an inclined surface that can carry a respective optical fiber, the inclined surfaces of the plurality of plate-shaped parts being inclined, relative to the extending direction of the rail, in the same direction. The plurality of plate-shaped parts are arranged side by side along the extending direction of the rail with the first surface of one plate-shaped part facing the second surface of an adjacent plate-shaped part and are contacted by the push-up part so as to move toward the inclined surface side.