A nucleic acid detection host includes a host body, a detection kit installation area, a sample heating area, a sampling groove, a heating structure, and an image collection unit. The detection kit installation area is configured to detachably install a nucleic acid detection kit. The sampling groove is disposed on the detection kit installation area and is connected to the detection kit installation area. The heating structure is configured to heat the nucleic acid detection kit to perform a PCR amplification reaction and an electrophoretic detection. The image collection unit is configured to collect an image of the nucleic acid detection kit. A nucleic acid detection device including the nucleic acid detection host is also disclosed. The nucleic acid detection device has a simple structure, which is portable, flexible, and convenient, and can be used at home.