A hooking and piercing device (1) for vials and tubes provided with a closing cap openable by pressure, comprising a hooking hood (2) with circumferentially distributed hooking toothed sectors (3) and an axial perforated stem (4) which extends axially and centrally out of the hooking hood (2) to be engaged by pressure inside a tube. The axial perforated stem (4) has an axially perforated appendix (6) which extends inside the hooking hood (2) and is shaped like a syringe luer. A frangible closing element (17) on the axial perforated stem (4) may be provided at the end of the axial perforated stem (4) opposite to the one with the luer-shaped appendix (6).