A paper separation structure arranged in an office machine, comprising: a separating roller set having a separating roller and a shaft, and a pick roller arranged to correspond to the separating roller set and contacted with the separating roller set, wherein the separating roller has a roller frame, a shaft hole is formed in the roller frame for accommodating the shaft, and a rubber layer connected with the pick roller, the shaft hole has a narrow inner diameter at a middle of the shaft hole and a wide inner diameter at opposite ends of the shaft hole to form two swing spaces between the middle of the shaft hole and opposite ends of the shaft hole, the roller frame and the rubber layer are swung about a joint between the middle of the shaft hole and the shaft.