This disclosure relates generally to medical tubing assemblies and methods for fluid delivery from a single fluid reservoir. In one example, a tubing assembly may be configured to couple to an endoscope for distributing fluid between the endoscope and a fluid reservoir during an endoscopic procedure. The assembly may comprise a tubing manifold, a cap configured to be removably attached to the fluid reservoir, a portion of gas supply tubing and lens wash supply tubing in fluid communication between the manifold and the endoscope, a portion of gas supply tubing in fluid communication between the cap and the manifold, and a portion of lens wash supply tubing in fluid communication between the fluid reservoir and the manifold. Further examples may include portions of irrigation supply tubing and portions of alternate gas supply tubing selectively connectable in fluid communication between the manifold, fluid reservoir and endoscope.