Invention Application
- Patent Title: METHOD FOR TARGET SPECIFIC RNA TRANSCRIPTION OF DNA SEQUENCES
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Application No.: US17724309Application Date: 2022-04-19
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Publication No.: US20220259649A1Publication Date: 2022-08-18
- Inventor: Keith Brown
- Applicant: JumpCode Genomics, Inc.
- Applicant Address: US CA San Diego
- Assignee: JumpCode Genomics, Inc.
- Current Assignee: JumpCode Genomics, Inc.
- Current Assignee Address: US CA San Diego
- Main IPC: C12Q1/6869
- IPC: C12Q1/6869 ; C12Q1/6865

Abstract:
Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target including using hairpin adaptors having a double stranded promoter and an overhang which hybridizes with a reverse-complementary overhang on a target nucleic acid. RNA transcription eliminates clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing.
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