公开(公告)号：US20230122979A1

公开(公告)日：2023-04-20

申请号：US17758659

申请日：2021-01-15

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12N15/10 ,  C12N9/16 ,  C12Q1/37 ,  C12Q1/6806

Abstract: Provided herein are methods of normalizing a population of nucleic acid samples. Methods herein can comprise: contacting a plurality of nucleic acid samples to a normalizing agent, wherein each nucleic acid of the plurality comprises a sample-specific barcode, and wherein the normalizing agent comprises a plurality of labeled enzymes capable of binding to each sample specific barcode; contacting the product to a capture agent to capture the nucleic acids that are bound to the normalizing agent; and treating the product with a protease to release the bound nucleic acids, thereby creating a normalized library having more even representation of each nucleic acid sample than the plurality of nucleic acid samples before normalization.

公开(公告)号：US10968536B2

公开(公告)日：2021-04-06

申请号：US15553495

申请日：2016-02-25

Applicant: JumpCode Genomics, Inc. ,  The Scripps Research Institute

Inventor： Keith Brown ,  Daniel R. Salomon ,  Steven Head ,  Azeem Siddique ,  Phillip Ordoukhanian

IPC: C40B40/08 ,  C12N15/10

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.

公开(公告)号：US20250084484A1

公开(公告)日：2025-03-13

申请号：US18728252

申请日：2023-01-10

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith BROWN

IPC: C12Q1/6886 ,  C12Q1/6855

Abstract: Provided herein are methods and compositions for analysis of a transcriptome, for example detection of a RNA isoform, of a cell, tissue, organ, or subject.

公开(公告)号：US20240182951A1

公开(公告)日：2024-06-06

申请号：US18553167

申请日：2022-03-30

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith BROWN ,  Jon ARMSTRONG ,  Azeem SIDDIQUE

IPC: C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

CPC classification number: C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

Abstract: Disclosed herein are compositions and methods related to the detection and/or elimination of a first nucleic acid and enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20230193401A1

公开(公告)日：2023-06-22

申请号：US18112239

申请日：2023-02-21

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith Brown

IPC: C12Q1/6886 ,  A61K49/00 ,  C12Q1/6869 ,  G16H15/00 ,  C12Q1/6809 ,  C12Q1/6883 ,  A61K31/7072 ,  A61K31/7056 ,  A61K38/45 ,  C12Q1/6876

CPC classification number: C12Q1/6886 ,  A61K31/7056 ,  A61K31/7072 ,  A61K38/45 ,  A61K49/0054 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Y201/01 ,  G16H15/00 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/178 ,  Y02A90/10

Abstract: Methods and compositions related to the use of Mobile Element Insertions and their adjacent genomic sequences. Methods using MEIs as markers for cellular proliferation, as targets for pharmaceuticals, as markers for tissue fingerprinting and in related methods and compositions are disclosed herein. Methods and compositions relate to the detection, treatment and ongoing monitoring of cell proliferation events, cancer, and deleterious effects of mobile elements in aging, and to the selection, use and monitoring of the success of treatment regimens to address these conditions.

公开(公告)号：US20220145359A1

公开(公告)日：2022-05-12

申请号：US17430102

申请日：2020-02-11

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12Q1/6806 ,  C12N9/22 ,  C12N15/11 ,  C12N15/10

Abstract: Disclosed herein are compositions and methods related to the elimination of a first nucleic acid and enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20210164128A1

公开(公告)日：2021-06-03

申请号：US17172029

申请日：2021-02-09

Applicant: JumpCode Genomics, Inc. ,  The Scripps Research Institute

Inventor： Keith Brown ,  Daniel R. Salomon ,  Steven Head ,  Azeem Siddique ,  Phillip Ordoukhanian

IPC: C40B40/08 ,  C12N15/10

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.

公开(公告)号：US10604802B2

公开(公告)日：2020-03-31

申请号：US15116404

申请日：2015-02-03

Applicant: JumpCode Genomics, Inc.

Inventor： Keith Brown ,  Peter Dansky

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6874 ,  C12Q1/6811

Abstract: Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US11761039B2

公开(公告)日：2023-09-19

申请号：US17506431

申请日：2021-10-20

Applicant: JumpCode Genomics, Inc.

Inventor： Keith Brown ,  Peter Dansky

IPC: C12Q1/6874 ,  C12Q1/6811 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1093 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6811 ,  C12Q2521/301 ,  C12Q2535/122

Abstract: Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20230265528A1

公开(公告)日：2023-08-24

申请号：US18041041

申请日：2021-08-11

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12Q1/689 ,  C12Q1/6869

CPC classification number: C12Q1/689 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Disclosed herein are compositions and methods related to the elimination of a first nucleic acid and/or enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.