The present disclosure relates to the field of biochip detection, and provides a biochip detection method, a biochip detection device, and an biochip detection apparatus. The biochip detection method includes: introducing a to-be-tested sample into a biochip, the biochip including a plurality of micro-reaction chambers; performing PCR amplification on the to-be-tested sample in the biochip; irradiating the biochip with excitation light rays at different intensities, and collecting images of the biochip under the excitation light rays at different intensities, the excitation light rays being used to excite a fluorescent probe in the to-be-tested sample to emit light; performing data processing on the collected images to obtain the quantity of positive micro-reaction chambers; and calculating the quantity of copies of the to-be-tested sample in accordance with the quantity of positive micro-reaction chambers.