There is provided a display substrate, in which, a first reference voltage line and a first reference voltage auxiliary line, which extend in different directions, are respectively disposed in one of a second wiring layer, a third wiring layer and a fourth wiring layer, a second reference voltage line extends in the same direction as the first reference voltage auxiliary line, the first reference voltage auxiliary line is electrically coupled to the first reference voltage line through via holes in an insulating layer therebetween, via a coupling line extending in the same direction as the first reference voltage line, the first reference voltage auxiliary line and the first reference voltage line provide a reset voltage to a first electrode of a first capacitor, and the second reference voltage line provides a reset voltage to a pixel electrode in a pixel electrode layer. A display device is further provided.