Invention Application
- Patent Title: Continuous Sample Purification Systems and Methods
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Application No.: US17861638Application Date: 2022-07-11
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Publication No.: US20220339554A1Publication Date: 2022-10-27
- Inventor: Scott P. Collins , Federico Carlos Rodriguez Peralta , Nephi D. Jones , Christopher D. Brau
- Applicant: Life Technologies Corporation
- Applicant Address: US CA Carlsbad
- Assignee: Life Technologies Corporation
- Current Assignee: Life Technologies Corporation
- Current Assignee Address: US CA Carlsbad
- Main IPC: B01D11/04
- IPC: B01D11/04 ; B01D11/02 ; B01D15/36 ; B01D15/38

Abstract:
Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.
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