The present disclosure relates to a digital microfluidic chemiluminescence detection chip, a detection method and a detection device. The digital microfluidic chemiluminescence detection chip includes a first baseplate and a second baseplate disposed oppositely. A cavity formed by the first and second baseplate includes a mixing and incubating area for combining an antigen, a magnetic particle antibody and an antibody, a luminescence detection area for chemiluminescence and detecting an optical signal, and a communication path for communicating the mixing and incubating area and the luminescence detection area. The first baseplate is provided with a drive array for driving sample solution to move and an optical sensing array for acquiring a luminescence signal of the sample solution. The drive array corresponds to positions of the mixing and incubating area, the luminescence detection area and the communication path. The optical sensing array corresponds to a position of the luminescence detection area.