A 3D display assembly, a display panel thereof and a method of manufacturing display panel. The display panel includes: a back plate, and a plurality of sub-pixel structures and partition structures on the back plate. In a first direction, each of the sub-pixel structures includes one or more first sub-sub-pixel structures and one or more second sub-sub-pixel structures arranged alternately. The first sub-sub-pixel structures have the same luminous color as the one or more second sub-sub-pixel structures. Each of the first sub-sub-pixel structures includes a first anode, and each of the second sub-sub-pixel structures includes a second anode. Second anodes are disposed at a side of the partition structures away from the back plate. Adjacent first anodes are partitioned by a partition structure. Orthographic projections of the first anodes on the back plate and orthographic projections of the second anodes on the back plate are connected, or the orthographic projections of the first anodes on the back plate and the orthographic projections of the second anodes on the back plate are overlapped and there is no non-light emitting region between sub-sub-pixel structures, therefore, no dark region is formed in a human eye observation region during 3D display, thereby solving moire problem.