There is provided an optical positioning system including a detected surface, an optical sensor, a register and a processor. The detected surface has interleaved bright regions and dark regions arranged in a transverse direction. The optical sensor captures an image frame of the detected surface within a field of view thereof and using a shutter time, wherein the detected surface and the optical sensor have a relative movement in the transverse direction. The register records a type of a last passed edge. The processor calculates a first position using a first algorithm upon the recorded last passed edge being a bright-to-dark edge and the field of view being aligned with one of the dark regions, and calculates a second position using a second algorithm, different from the first algorithm, upon the recorded last passed edge being a dark-to-bright edge and the field of view being aligned with the same one of the dark regions.