Provided are a display substrate, a preparation method thereof, and a display device. The display substrate includes a display region and a bonding region on one side of the display region. The bonding region at least includes a lead area; the display region includes a plurality of data lines and a plurality of data fanout lines, the lead area includes a plurality of lead wires, and orthographic projections of the plurality of data lines and the plurality of data fanout lines on a plane of the display substrate are at least partially overlapped. At least one lead wire is connected to the data line through the data fanout line. In the lead area, an orthographic projection of any one of the lead wires on the plane of the display substrate and orthographic projections of other lead wires on the plane of the display substrate have no overlap region.