- 专利标题： METHOD FOR PRODUCING CARTILAGE TISSUE
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申请号： US18003737申请日： 2021-07-01
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公开(公告)号： US20230257686A1公开(公告)日： 2023-08-17
- 发明人: Takeshi TAKARADA , Ryosuke IWAI , Kohei SUZUKI , Natsuki FUKASAWA
- 申请人： NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY , KAKE EDUCATIONAL INSTITUTION , NISSAN CHEMICAL CORPORATION
- 申请人地址： JP Okayama
- 专利权人： NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY,KAKE EDUCATIONAL INSTITUTION,NISSAN CHEMICAL CORPORATION
- 当前专利权人： NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY,KAKE EDUCATIONAL INSTITUTION,NISSAN CHEMICAL CORPORATION
- 当前专利权人地址： JP Okayama; JP Okayama; JP Tokyo
- 优先权： JP 20115594 2020.07.03
- 国际申请： PCT/JP2021/024967 2021.07.01
- 进入国家日期： 2022-12-29
- 主分类号： C12M3/00
- IPC分类号： C12M3/00 ; C08F220/04 ; C08F220/34 ; C12N5/07
摘要：
The present invention is to provide a method for producing a cartilage tissue which comprises a step of providing a substrate for producing cell aggregates provided with a plurality of spots comprising a copolymer containing recurring units derived from monomers represented by the following formulae (I) and (II):
[wherein Ua1, Ua, Ra1, Ra2 and Rb are as described in the specification and claims] on a substrate having an ability to suppress adhesion of cells; a step of seeding human cartilage progenitor cells which are positive for PRRX1 protein and derived from pluripotent stem cells on the substrate; a step of producing cell aggregates by culturing the cells; and a step of culturing the aggregates to produce a cartilage tissue.
[wherein Ua1, Ua, Ra1, Ra2 and Rb are as described in the specification and claims] on a substrate having an ability to suppress adhesion of cells; a step of seeding human cartilage progenitor cells which are positive for PRRX1 protein and derived from pluripotent stem cells on the substrate; a step of producing cell aggregates by culturing the cells; and a step of culturing the aggregates to produce a cartilage tissue.
公开/授权文献
- US2155314A Battery tester 公开/授权日：1939-04-18
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