The presently claimed and described technology provides integrated automated instruments (100, 100A, 100B, 100C) and methods (800) for automatically processing a whole blood sample (50) in a single sample tube (80). These include: mixing the sample in the tube; analyzing a portion (50P) of the mixed sample (50M); centrifuging the sample in the tube thereby separating plasma (60); and analyzing a portion (60P) of the plasma. A pretreatment module (200) may mix, centrifuge, transport, and store the sample. The integrated instrument may include multiple detector components and does not require external sample handling.