Invention Publication

  • Patent Title: A DFL-PRODUCING STRAIN
  • Application No.: US18258770
    Application Date: 2021-12-21
  • Publication No.: US20240043891A1
    Publication Date: 2024-02-08
  • Inventor: Margit PEDERSEN
  • Applicant: GLYCOM A/S
  • Applicant Address: DK Hørsholm
  • Assignee: GLYCOM A/S
  • Current Assignee: GLYCOM A/S
  • Current Assignee Address: DK Hørsholm
  • Priority: DK 202001450 2020.12.22
  • International Application: PCT/EP2021/086932 2021.12.21
  • Date entered country: 2023-06-21
  • Main IPC: C12P19/18
  • IPC: C12P19/18 C12N9/10
A DFL-PRODUCING STRAIN
Abstract:
The present invention relates to a genetically modified cell expressing an α-1,2-fucosyltransferase and an α-1,3-fucosyltransferase, and a transporter protein of the major facilitator superfamily (MFS) and to a method for recombinant production of human milk oligosaccharides (HMOs) using said genetically modified cell. More particularly, the invention provides a method for recombinant production of and a genetically modified cell capable of producing difucosyllactose (DFL) as the most abundant HMO, with a relatively low content of 3-fucosyllactose (3FL) and/or 2′-fucosyllactose (2′FL).
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