The present invention relates to a biosensor for inspecting the susceptibility of a microorganism to an antibiotic, a fabrication method therefor, and a method for inspecting the susceptibility of a microorganism to an antibiotic by using same, wherein the biosensor comprises: a substrate including polyethylene terephthalate; and a sensor part formed on one surface or both surfaces of the substrate and comprising an electrode inclusive of a first electrode and a second electrode which interdigitate with each other.