A visualization system and methods for simultaneously visualizing white light and visible fluorescent light, the visualization involving an illumination system and an imaging system operable with the illumination system. The illumination system has a light source produces illumination light (white light and fluorescence excitation light), an optical notch rejection filter to remove light having peak fluorescence excitation wavelengths from the illumination light, and illumination optics to at least one of deliver and condense the filtered illumination light. The imaging system has imaging optics, a fluorescence excitation light removal filter to remove excitation light from the imaged light, a beam splitter to split the filtered images light into a first beam (white light), and a second beam (fluorescent imaging light), an optical notch rejection filter to ensure that light, having peak fluorescence emission wavelengths of a fluorophore, does not reach a white light camera, an optical notch pass filter to pass only the fluorescent imaging light component, a camera to image the white light; and another camera to image the fluorescent light.