The present invention pertains to a liquid handling device that comprises a laminate of a first member and a second member. This liquid handling device is provided with a reservoir that is a space for pooling a liquid and disposed between the first and second members, and a plurality of wells that are plurality of first recesses disposed on the surface of the first member facing the second member. The first and/or second members have flexibility. The first and second members are not bonded between the reservoir and the plurality of wells. When a part of the first or second member, said part corresponding to the reservoir, is pressed in the state where a liquid is pooled in the reservoir, the liquid in the reservoir moves between the first and second members toward the plurality of wells.