A method of analyzing a lubricating oil may include: measuring a first concentration of components in a lubricating oil prior to introduction into a cylinder in a two-stroke engine using x-ray fluorescence, the components comprising: sulfur and total calcium; collecting a scrape-down lubricating oil that corresponds to the lubricating oil after having been passed through the cylinder during fuel combustion of a fuel comprising 0.5 wt % or less sulfur; measuring a second concentration of the components in the scrape-down lubricating oil using the x-ray fluorescence; and calculating an amount of acid neutralized during combustion based on the first and second concentration of the components. Based on said analysis a change to a property of the scrape-down lubricating oil and/or changing a feed rate of the lubricating oil into the cylinder based on the amount of acid neutralized may be implemented.