Disclosed is an application of a class of selenium-containing organic compounds in copper sulfide flotation collectors, including organic compound with selenium atoms as functional groups and containing single selenium atom or multiple selenium atoms, where the organic compound with selenium atoms as functional groups and containing single selenium atom or multiple selenium atoms includes but not limited to selenophene, phenylselenol and diselenide as copper collectors; and a class of organic compound containing selenium atoms combined with nitrogen, oxygen, phosphorus, sulfur and carbon, where the class of organic compound containing selenium atoms combined with nitrogen, oxygen, phosphorus, sulfur and carbon includes but not limited to selenomethyl ester, carbonyl selenomethyl ester, oxycarbonyl selenomethyl ester, selenourea, selenium nitrogen, selenoxanthonic acid, selenoxanthate, selenophosphoric acid, selenium phosphate ester, triphenylphosphine selenide, all kinds of benzoselenediazole molecules as copper collectors.