Invention Publication
- Patent Title: METHODS OF ISOTHERMAL COMPLEMENTARY DNA AND LIBRARY PREPARATION
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Application No.: US18475885Application Date: 2023-09-27
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Publication No.: US20240150753A1Publication Date: 2024-05-09
- Inventor: Allison Yunghans , Angelica Marie Barr Schalembier , Kayla Busby , Stephen M. Gross , Robert Scott Kuersten , Frederick W. Hyde
- Applicant: Illumina, Inc.
- Applicant Address: US CA San Diego
- Assignee: Illumina, Inc.
- Current Assignee: Illumina, Inc.
- Current Assignee Address: US CA San Diego
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12N9/12 ; C12N9/22
Abstract:
Described herein are compositions and methods for preparing double-stranded complementary DNA (cDNA) from RNA. In some embodiments, these methods allow isothermal preparation of cDNA. In some embodiments, these methods allow mesophilic or thermostable preparation of cDNA. Also described herein are compositions and methods for preparing cDNA and a library of double-stranded cDNA fragments in a single reaction vessel.
Information query
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