A microfluidic substrate has a first straight region, a second straight region, and a first turning region, which is substantially of a ring sector and includes a first arc edge and a second arc edge that are opposite. The microfluidic substrate includes first straight driving electrodes in the first straight region, second straight driving electrodes in the second straight region, and turning driving electrodes the first turning region. A border of each turning driving electrode includes at least one first reference point coinciding with the first arc edge and at least one second reference point coinciding with the second arc edge. A radius of the first arc edge is greater than or equal to (√{square root over (3)}−1) times of a first dimension of a reference electrode, and a radius of the second arc edge is greater than or equal to 3/2 times of the first dimension of the reference electrode.