Systems and methods for amplification and detection of metabolite signals are provided. A plurality of files containing m/z signal intensities may be captured by a mass spectrometer. Each file of m/z signal intensities may include signals associated with mass measurements of compounds in a respective sample. The datasets of the chromatograms may be combined into a merged spectra of m/z signal intensities. A concentration of signals may be identified in the merged chromatogram as following a specified statistical distribution and determined to be indicative of a metabolite when the concentration of signals corresponds to one or more mass measurements associated with a metabolite and an isotopologue of the metabolite.