The present invention provides a biodegradable multilayer film, a manufacturing method therefor, and an eco-friendly packaging material containing same, the biodegradable multilayer film comprising a base layer and a biodegradable resin layer, wherein the biodegradable resin layer includes a polyhydroxyalkanoate (PHA) resin, and the thermal bonding strength of the biodegradable resin layer is 0.5 kgf/15 mm to 15 kgf/15 mm, the water permeability is 3 g/m2·atm·day or less, and the oxygen permeability is 10 cc/m2·atm·day or less. The biodegradable multilayer film can be biodegraded in natural conditions such as in soil and in the ocean, can improve barrier properties against moisture and oxygen without including aluminum or nylon materials, and can further improve adhesive properties. As such, the biodegradable multilayer film can be used as a packaging material in various fields such that a high-quality and eco-friendly packaging material is provided.