- 专利标题: METHODS AND CONSTRUCTS FOR PRODUCTION OF LENTIVIRAL VECTOR
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申请号: US18680137申请日: 2024-05-31
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公开(公告)号: US20240336936A1公开(公告)日: 2024-10-10
- 发明人: Young SHIN , Anandita SETH , Bingnan GU
- 申请人: LONZA WALKERSVILLE, INC.
- 申请人地址: US MD Walkersville
- 专利权人: LONZA WALKERSVILLE, INC.
- 当前专利权人: LONZA WALKERSVILLE, INC.
- 当前专利权人地址: US MD Walkersville
- 分案原申请号: US17001114 2020.08.24
- 主分类号: C12N15/867
- IPC分类号: C12N15/867 ; C07K14/16 ; C12N5/071 ; C12N9/12 ; C12N15/86
摘要:
The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.
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