A light-emitting device comprises a first electrode, at least two light-emitting units and a second electrode which are sequentially stacked in a first direction. The at least two light-emitting units comprise a first light-emitting unit and a second light-emitting unit, the second light-emitting unit being located between the first light-emitting unit and the second electrode. At least one light-emitting unit comprises a light-emitting layer and an exciton blocking layer located on the side of the light-emitting layer close to the first electrode; the exciton blocking layer comprises a first sub-layer and a second sub-layer which are stacked in the first direction; the first sub-layer is located between the second sub-layer and the light-emitting layer; and in the first direction, the thickness of the first sub-layer is smaller than that of the second sub-layer, and the highest occupied molecular orbital energy level of the first sub-layer is higher than the highest occupied molecular orbital energy level of the second sub-layer. The light-emitting device provided by the present disclosure can enable exciton recombination regions of the light-emitting layer corresponding to each light-emitting unit to get close as much as possible, thereby improving light-emitting efficiency of the light-emitting device.