A method is provided for detecting tampering of a printed circuit board (PCB) using a tamper detection circuit implemented in an integrated circuit (IC) mounted on the PCB. In the method, a reference signal is generated and provided to a terminal of the IC. The terminal is connected to another circuit mounted on the PCB. A first response signal is received in response to the reference signal at the terminal. One or more characteristics of the first response signal are stored in a memory. The reference signal is again provided to the terminal of the integrated circuit and a second response signal is received at the terminal. The stored characteristics of the first response signal are compared to corresponding characteristics of the second response signal to generate a comparison result. The comparison result is used to detect tampering. When tampering is detected, an indication is provided.