A display substrate is provided. The display substrate includes a quantum dots layer having a plurality of quantum dots. The quantum dots layer includes a plurality of quantum dots blocks respectively in a plurality of first apertures. A respective quantum dots block of the plurality of quantum dots blocks includes a first region and a second region. A concentration of a respective product of a respective reactant produced by a respective reaction in the first region is greater than a concentration of the respective product of the respective reactant produced by the respective reaction in the second region. The respective reaction is one of a dimerization, oligomerization, polymerization, a condensation reaction, or any combination thereof.