Invention Application
- Patent Title: METHOD FOR PRODUCING SUGAR INCLUDING LACTO-N-TRIOSE II AS CORE TRISACCHARIDE AND METHOD FOR PRODUCING CRYSTALS OF SAID SUGAR
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Application No.: US18723065Application Date: 2022-12-21
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Publication No.: US20250051814A1Publication Date: 2025-02-13
- Inventor: Tomotoshi SUGITA , Sotaro SANPEI
- Applicant: KYOWA HAKKO BIO CO., LTD. , KIRIN HOLDINGS KABUSHIKI KAISHA
- Applicant Address: JP Tokyo; JP Tokyo
- Assignee: KYOWA HAKKO BIO CO., LTD.,KIRIN HOLDINGS KABUSHIKI KAISHA
- Current Assignee: KYOWA HAKKO BIO CO., LTD.,KIRIN HOLDINGS KABUSHIKI KAISHA
- Current Assignee Address: JP Tokyo; JP Tokyo
- Priority: JP2021-207590 20211221
- International Application: PCT/JP2022/047244 WO 20221221
- Main IPC: C12P19/28
- IPC: C12P19/28 ; C07K14/245 ; C12N1/20 ; C12R1/19

Abstract:
The present invention relates to a microorganism having a reduced or inactivated activity of a protein according to any one of the following [1] to [3] and improved productivity of lacto-N-triose II or an oligosaccharide having a lacto-N-triose II (LNTII) skeleton as compared with a parent strain: [1] a protein consisting of the amino acid sequence represented by SEQ ID NO: 2 or 4, [2] a mutant protein having an oligosaccharide transporting activity and consisting of an amino acid sequence in which 1 to 20 amino acids are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO: 2 or 4, and [3] a homologous protein having an oligosaccharide transporting activity and consisting of an amino acid sequence having an identity of 90% or more with the amino acid sequence represented by SEQ ID NO: 2 or 4.
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