Methods and apparatus for illuminating a biometric aspect, such as periocular region features or iris features, with multiple illumination configurations to create multiple illumination channels for computer analysis are disclosed. Multiple illumination channels may be used for quality assurance by comparing the actual illumination channel property of a given image to the illumination channel that was activated for image capture. Images captured at the multiple illumination channels can also be used in biometric authentication by providing additional information for comparison to a stored template generated from images taken at multiple illumination channels during enrollment. Biometric authentication using a quality assurance process may be resistant to attempts to bypass biometric authentication.