The invention relates to novel 2 Beta ,16 Beta -diamino-17unsubstituted-androstanes, to acid addition salts and quaternary ammonium derivatives of these compounds and to processes for their preparation. The compounds have very interesting biological activities. The mono- and bis-quaternary ammonium salts of the above compounds have neuromuscular blocking activity. The 2 Beta , 16 Beta diamino-3 Alpha -hydroxy(or 3 Alpha -acyloxy)-17-unsubstitutedandrostanes according to the invention are neuromuscular blocking agents having a quick onset and short duration of action.