发明授权
- 专利标题： Large scale purification of hepatitis type B antigen using polyethylene glycol
- 专利标题（中）： 使用聚乙二醇大规模纯化乙型肝炎抗原
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申请号： US426825申请日： 1973-12-20
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公开(公告)号： US3951937A公开(公告)日： 1976-04-20
- 发明人: John Vnek , Alfred M. Prince
- 申请人： John Vnek , Alfred M. Prince
- 申请人地址： NY New York
- 专利权人： The Community Blood Council of Greater New York, Inc.
- 当前专利权人： The Community Blood Council of Greater New York, Inc.
- 当前专利权人地址： NY New York
- 主分类号： C07K14/02
- IPC分类号： C07K14/02 ; C07G7/00
摘要：
Highly purified type B hepatitis antigen (HB Ag) is produced from fluid blood material containing such antigen by subjecting blood material containing naturally occurring HB Ag to a double precipitation with polyethylene glycol. In each precipitation, the pH of the fluid blood material is maintained at approximately 4.4 to 4.7 and a polyethylene glycol concentration of approximately 4.0 to 4.5 weight per cent is used. Particularly good results are obtained if the temperature of the material is maintained in the range of 0.degree. to 8.degree.C after the polyethylene glycol has been added. The antigen thus obtained may be further purified by absorption of impurities to hydroxy apatite, followed by isopycnic banding and zonal ultracentrifugation. Hydroxy apatite column chromatography is used to separate the purified antigen into three separate populations of particles.
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