发明授权
- 专利标题： DNA Joining method
- 专利标题（中）： DNA连接法
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申请号： US898887申请日： 1978-04-21
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公开(公告)号： US4264731A公开(公告)日： 1981-04-28
- 发明人: John Shine
- 申请人： John Shine
- 申请人地址： CA Berkeley
- 专利权人： The Regents of the University of California
- 当前专利权人： The Regents of the University of California
- 当前专利权人地址： CA Berkeley
- 主分类号： C07K14/62
- IPC分类号： C07K14/62 ; C12N15/00 ; C12P19/34 ; C12R1/19
摘要：
A selected portion of DNA molecules having reactant ends which are capable of being joined in a ligase catalyzed reaction are pretreated so a to remove the 5'-terminal phosphate groups. Such a treatment reduces the frequency of joining an undersired combination and enhances the frequency of joining the desired combination.
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