Easily split enzyme substrates for the quantification of proteases having the general formulaR.sub.1 --p-Glu--A.sub.1 --A.sub.2 --NH--R.sub.2,whereR.sub.1 =H or a protective group, preferably t-butyloxycarbonyl, benzyloxycarbonyl;A.sub.1 =Gly, Ala, Val, Leu, Ile, Ser, Thr, Pro, Pip, Phe or Tyr;A.sub.2 =Arg or Lys;R.sub.2 =an aromatic, possibly substituted, hydrocarbon group, wherein --NH--R.sub.2 is a physico-chemically determinable group, preferably a chromogenic or fluorogenic group which is split by a present enzyme and then forms a cleavage product of the formula H.sub.2 N--R.sub.2 the amount of which can be quantified.Processes for the production of said substrates. Method in the laboratory diagnostics of proteases using said substrates.